Figure 1
From: Functional divergence and origin of the DAG-like gene family in plants
Maize DAL proteins and their conserved motifs. (a) Multiple sequence alignment of maize DAL proteins was carried out using ClustalW2.0, and the NJ tree was built using MEGA v5.0. The chloroplast and mitochondrial transit peptides of maize DAL proteins were predicted using Predotar (http://urgi.versailles.inra.fr/predotar/predotar.html) and (/) TargetP (http://www.cbs.dtu.dk/services/TargetP/). M, mitochondria; C, chloroplast. The DAL proteins marked by an asterisk were observed in the plastid proteome. (b) Alignment of conserved DAL domains in maize DAL proteins was conducted using ClustalW2.0 and displayed with GeneDoc. The secondary structure of the DAL domain was inferred using MINNOU (http://minnou.cchmc.org/). (c,d) Putative motifs were explored using the MEME server with the parameters of between 10 and 50 aa in length and sharing of each motif among all ZmDAL proteins.
