Figure 1 | Scientific Reports

Figure 1

From: Sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta

Figure 1

Sca-1 was determined to be highly expressed in undifferentiated TS cells in culture by Northern blot and qRT-PCR (A) and FACS analysis (B). Day 0/0 represents undifferentiated, proliferating TS cells while 2, 4, 6 and Day 2, Day 4 and Day 6 represent cultures differentiated for 2, 4 or 6 days. Sca-1 was also expressed in TS cells in culture as detected by immunofluorescence (C; +FGF) however became undetectable following differentiation (C; -FGF). Immunofluorescence in cells (C) in the presence and absence of FGF matched the pattern of expression observed by FACS (B), however was not obvious in as a high frequency (+FGF) which is likely due to the more sensitive detection of Sca-1 expression by FACS. Scale bar = 100 um. Northern blot results shown in (A) are cropped and organized to be presented as a composite from multiple blots/films.

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