Figure 1
From: Development and Characterization of A Novel Prox1-EGFP Lymphatic and Schlemm’s Canal Reporter Rat
Characterization of the reporter expression in lymphatic vessels of Prox1-EGFP rat. (A) EGFP expression patterns in adults: (i,ii) Bright light and fluorescence headshots of Prox1-EGFP mouse (left) and Prox1-EGFP rat (right) highlight the size difference between the two rodents and the strong reporter expression in their lenses. (iii–iv) Cervical and axillary lymph nodes (arrowheads), which are difficult to locate under bright light without a contrasting dye, could be easily identifiable under fluorescence in Prox1-EGFP rats. (v–vi) Fluorescent images showing lymphatic vessels in the capsular and subcapsular area of an adult lymph node. (vii–xvi) Lymphatic vessels and their luminal valves (arrowheads) are clearly visible in the bladder (vii–viii), mesentery (ix–x), and testicle (xi–xii). Schlemm’s canal (arrowhead, xiii) and limbal lymphatic vessels (arrowhead, xiv) of the same eye imaged at different focuses show strong EGFP expression. Scale bars: 500 \({\rm{\mu }}\)m (v–xii), 100 µm (xiii–xiv). (B) EGFP expression patterns in embryos: (i–iii) Compared to wild type embryo (left), Prox1-EGFP rat embryo (right) shows a strong EGFP expression in the lens, central nervous system, and liver. (iv–vi) Dermal capillary lymphatic network could be easily visualized in the back skin of embryo (E17.5). Panels (v) and (vi) are enlarged images of (iv). (vii–xxi) Bright field and fluorescence images showing lymphatic vessels in the subcutaneous area (vii–ix), thoracic cavity (x–xii), diaphragm (xiii–xv), mesentery (xvi-xviii), and bladder (xix–xxi). Scale bars: 500 µm.
