Figure 6
From: Pattern of retinal morphological and functional decay in a light-inducible, rhodopsin mutant mouse
Glial activation. (A,B) Macro and microglia in the retina of Tvrm4 mice exposed to 12,000 lux for 1 min and examined after 2 days. (A) whole mount ICCH with Iba1 antibodies (green) labels activated microglial cells (arrows) in the outer retina. Red staining: GFAP. (B) Pycnotic nuclei of dying photoreceptors (ethidium staining, red) are observed in the ONL (arrowheads) and inside phagocytic microglial cells. (C) vertical section stained with antibodies against GFAP (red staining), which highlights the radial processes of activated Muller cells (arrows). Nuclei are stained blue with Hoechst. (D) whole mount retina of a Tvrm4 mouse, observed 7 days after exposured. GFAP staining shows hypertrophic processes of astrocytes (A) in the corresponding retinal layer, intermingled with Muller cell endfeet.
