Figure 7
From: Pattern of retinal morphological and functional decay in a light-inducible, rhodopsin mutant mouse
Long-term effects of phenotype induction. Whole mount retinas exposed for 1 min to 12,000 lux and examined after 30 days. (A) Focus on cone outer segments, stained red by cone opsin antibodies, show that the central, dark area, has decreased compared to what shown in Fig. 3A. ONH: optic nerve head. (B) Vertical section of an eye cup from a Tvrm4 mouse similar to that shown in A. Cone arrestin (red) and rhodopsin staining (green) show that the photoreceptor-free zone (between the white arrows) remained restricted to the central retina, similarly to what shown in Fig. 2A. (C) Cone progressive shortening toward the central retina (on the left side of the picture), where a faint rhodopsin staining becomes visible. (D) Light sensitive channel (green) highlights punctuate staining in the outer retina (arrowheads), likely corresponding to re-growing outer segments of rods. Red: ethidium nuclear counterstaining.
