Figure 2 | Scientific Reports

Figure 2

From: Physiological stress-induced corticosterone increases heme uptake via KLF4-HCP1 signaling pathway in hippocampus neurons

Figure 2

Mouse hippocampus nerve cells have the capacity to uptake heme via HCP1. (A) Iron content in cells treated with different concentrations of heme for 2 h and with 30 μM heme for different time. (B) To visualize hemin accumulation in neurons, cells were incubated with 30 µM ZnPPIX for 2 h, and the fluorescence in ZnPPIX treated cells was apparently intensified and compared with the control. (C)The expression of HCP1 was decreased when cells were transfected with HCP1 siRNA, and the uptake of heme was correspondingly decreased. (D) The expression of HCP1 was increased when cells were transfected with HCP1 expression plasmid, and the uptake of heme was correspondingly increased. Values are means ± SD. *p < 0.05; **p < 0.01; ***p < 0.001.

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