Figure 3 | Scientific Reports

Figure 3

From: Physiological stress-induced corticosterone increases heme uptake via KLF4-HCP1 signaling pathway in hippocampus neurons

Figure 3

Corticosterone up-regulates HCP1 in HT-22 cells. (A) RT-PCR analysis showed that HCP1 mRNA expression was elevated when HT-22 was incubated with 15 μM and 30 μM CORT for 24 h. (B) HCP1 mRNA expression in HT-22 cells treated with 30 μM CORT for different times, and the HCP1 expression was increased after incubated for 4 h. (C) HCP1 protein expression in cells treated with different concentrations of corticosterone for 24 h, and the expression was increased when cells were treated with 10 μM corticosterone above. (D) HCP1 protein expression in cells treated with 30 μM corticosterone for different times, and the expression was increased after an 8 h incubation period. Values are means ± SD. *p < 0.05; **p < 0.01; ***p < 0.001.

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