Figure 2 | Scientific Reports

Figure 2

From: Random mutagenesis analysis and identification of a novel C2H2-type transcription factor from the nematode-trapping fungus Arthrobotrys oligospora

Figure 2

Identification and verification of the insertion of the hph cassette in transformants X5. (A) The sketch map of the insertion of the pMD-hph in transformants X5. The frame filled with diagonal lines presented the insertion of pMD-hph, and the X5inserF and X5inserR indicated the location of primers used for the PCR verification in Fig. 2C. (B) The schematic diagram of the location of gene 76g273 and the pMD-hph in X5, and restriction enzymes (SalI and HindIII) used for Southern blotting analysis were also indicated. The inserted site of the pMD-hph was set to 0, and the expected size of band hybridizing to the hph cassette in the X5 was 3189 bp and 1718 bp after digested by SalI and HindIII, respectively. (C) Verification of the insertion of the hph cassette in transformants X5 by PCR amplification using primers X5inserF/X5inserR. M, DNA marker, ladders are 5 kb, 3 kb, 2 kb, 1.5 kb, 1 kb, 750 bp, 500 bp, 250 bp and 100 bp. (D) Southern blotting analysis of the WT and X5 using restriction enzymes SalI and HindIII.

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