Figure 6
Transcriptional analysis of the gene 76g274 in A. oligospora under different conditions. β-tubulin gene (AOL_s00076g640) of A. oligospora was used as the internal control. (A) The transcription of gene 76g274 in A. oligospora during the growth of A. oligospora in TG medium. *P < 0.05 versus 1st day. (B) The transcription of gene 76g274 under various stress-tolerant conditions (TG broth as basic medium). WT, the control without treated; 42 °C and 12 °C, the mycelia were incubated at 42 °C or 12 °C for 1 h; pH 4 and pH 10, the mycelia were cultured in medium of pH 4 or pH 10 for 4 h; NaCl, the mycelia were treated by 0.5 M NaCl for 4 h; DS, sorbitol (0.5 M); SDS, sodium dodecyl sulfate (0.02%); CongRed, congo red (100 µg/mL); H2O2, H2O2 (5 mM); Vc, vitamin c (1 mg/mL); Ethanol, 2% ethanol; DMSO, dimethylsulfoxide (2%); Cu, CuSO4 (5 mM); Mn, MnSO4 (5 mM); Fe(II), FeSO4 (5 mM); Zn, ZnSO4 (5 mM); G418, geneticin sulfate (300 µg/mL); Carben, carbendazim (300 µg/mL); Hyg, hygromycin B (200 µg/mL); Actidione, 200 µg/mL actidione. The mycelia were treated at above condition for 4 h, respectively. *P < 0.05 versus WT. The horizontal bars depict the median, and data are presented as suggested by Weissgerber et al.58 and Klaus59.
