Figure 2

HuR/TTP axis protein levels are altered in patients with endometriosis: characterization using ELISA and western blotting in endometriosis patients and in normal fertile controls. Expression of HuR was significantly higher in eutopic endometrium compared to matched ectopic lesions from endometriosis patients as well as normal endometrium from healthy controls (Panel A). TTP protein was significantly higher in eutopic and ectopic matched endometrium from endometriosis patients compared to normal endometrium from healthy controls (Panel B). In a different cohort of women with endometriosis receiving dienogest, HuR and TTP protein was significantly higher in matched eutopic endometrium compared to ectopic lesions (Panels C,D, densitometry as ratio to ACTB and cropped blots are displayed. Full blot images are shown in Supplemental Figs 1 and 2). Expression data illustrated as mean ± SEM. *P < 0.05. Panels A,B (Greenville patient subset) consisted of n = 22 human eutopic endometrium, n = 22 human ectopic endometriotic lesions, n = 16 normal control endometrium. Panels C,D (Ottawa patient subset) consisted of n = 8 human eutopic endometrium and n = 8 human ectopic endometriotic lesions.