Figure 4

Upregulation of HuR/TTP axisRNA binding proteins Zfp36, Zfp36l1, Zfp36l2 (TTP family), and Elavl1 (HuR) mRNA in an in vivo mouse model of endometriosis. mRNA transcripts encoding for TTP (Zfp36) is elevated in ectopic compared to eutopic endometrium obtained from Balb/c mice (panel A). Zfp36L1 was elevated in ectopic compared to eutopic endometrium using matched t-test (panel C), however did not significantly differ by unpaired student’s t-test (panel B). mRNA expression for Elavl1 and Zfp36l2 was not statistically significant (Panel D,E; p = 0.09), and did not differ by paired/matching tests. Endometriosis was induced in BALB/cByJ mice by surgical implantation of endometrium isolated from donor mice (n = 2) into the left peritoneal cavity of recipient mice. Data presented consists of Days 7 (n = 3) and 17 (n = 3) of eutopic endometrium and ectopic lesion harvest. Expression patterns did not change by collection day, however data is presented as both time points pooled n = 6. Expression data illustrated as mean ± SEM. *P < 0.05.