Figure 5

TTP knockdown results in endometriotic (12Z) and endometrial epithelial (EECC)-specific effects in other RNABPs of HuR/TTP axis: Influence of TTP knockdown on TIS11 family and HuR RNA binding proteins in human endometrial epithelial (EECC) and endometriotic (12Z) cells. Transcript ZFP36 which encodes for TTP was significantly lower in both EECC and 12Z cells (Panels A,E). Transcripts for ZFP36L1 and ELAVL1 were also downregulated in EECC cells treated with TTP siRNA (panels B,D). The TTP family member ZFP36L2 did not statistically differ upon knockdown of TTP in EECCs (panel C). On the other hand, transcripts for ZFP36L2 and ELAVL1 were upregulated in 12Z cells treated with TTP siRNA (panels G,H). Transcripts for ZFP36L1 do notstatistically differ when knocked down with TTP siRNA in 12Zs (panel F). Expression data illustrated as mean ± SEM. *P < 0.05. Each group (treated and control) consisted of three wells performed in triplicates. Data is derived from three independent experiments; n = 3.