Figure 6

TTP knockdown results in distinct endometriotic (12Z) and endometrial epithelial (EECC)-specific inflammatory profiles: In vitro downstream mRNA analysis of inflammatory cytokines associated with RNA binding protein regulation in TTP knockdown EECCs and 12Zs. Transcripts for IL-6, TNF-α, HIF-1α were downregulated in EECC cells treated with TTP siRNA (Panels A,B,D) compared to scrambled controls. Transcripts for COX-2, GM-CSF, and IL-10 do not differ in TTP knockdown EECCs (Panels C,E,F). On the other hand, TTP knockdown results in upregulation of COX-2, HIF-1α and GM-CSF in endometriotic epithelial 12Z cells treated with TTP siRNA compared to scrambled controls (Panels I–K). Transcripts for IL-6, TNF-α, and IL-10 do not differ in TTP knockdown 12Zs (Panels G,H,L). Expression data illustrated as mean ± SEM. *P < 0.05. Each group (treated and control) consisted of three wells performed in triplicates. Data is derived from three independent experiments; n = 3.