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Figure 1

From: HIF1α regulates glioma chemosensitivity through the transformation between differentiation and dedifferentiation in various oxygen levels

Figure 1

GSCs exhibited higher apoptosis rates than differentiated cells. (A) Sorted GL261 and U87 CD133+/CD15+/NESTIN+ GSCs were cultured in stem cell medium, and these cells grew with a sphere morphology in suspension. (B) U87 neurospheres highly expressed CD133, CD15 and NESTIN. (C,D) There was an increased expression of ABCG2 and MGMT in U87 neurospheres. (E) Three to five-fold higher expression levels of ABCG2 and MGMT were observed for GL261 and U87 CD133+/CD15+/NESTIN+ GSCs than CD133−/CD15−/NESTIN− cells (* P < 0.05, Paired-samples T Test). (F) GL261 and U87 CD133+/CD15+/NESTIN+ GSCs arrested the cell cycle in G0/G1 (* P < 0.05, Paired-samples T Test). (G) Higher apoptosis rates were observed for GL261 and U87 CD133−/CD15−/NESTIN− cells than for GSCs after TMZ (100 μM) treatment (* P < 0.05, Paired-samples T Test).

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