Table 2 Primer sequences for qRT-PCR analysis.

From: Dietary arginine affects the insulin signaling pathway, glucose metabolism and lipogenesis in juvenile blunt snout bream Megalobrama amblycephala

Primer

Sequence Information

Forward primer (5′-3′)

Reverse primer (5′-3′)

β-actin

TCGTCCACCGCAAATGCTTCTA

CCGTCACCTTCACCGTTCCAGT

Akt1

GCTGGGTAAAGGCACGTTTG

CTCTCGGTGACCGTATGAGC

GS2

TTACACGGTCATTGCGTCCA

GACACAGCTCAGTCGGTGAA

PEPCK3

TCGCCTGGATGAAGTTCGAC

GTCTTGGTGGAGGTTCCTGG

IRS-14

AACCTGGTTGGCATCTACCG

ATCAGCTGGAGCACGATAGC

G6PDH5

TGGAGAAACCTTTTGGCCGT

CTGGGTACCAAACGGCTCTT

GK6

GCTTCCACTGGGATTCACCT

CGACGTTATTGCCTTCAGCG

PK7

CGAGATTGAGAACGGAGGCA

GTCCTTCTCAGACACTGCGG

FAS8

GTTTGCCAACCGCTTGTCTT

GGCCATGGCGAATAGCATTG

ACC9

TAGCAGTGAGCATTGGCACA

CATCGCTGGCGTATGAGGAT

GLUT210

CGGTGAAACCGAACAGGAGT

TTCTTTGAGATCGGGCCTGG

G6P11

TTCAGTGTCACGCTGTTCCT

TCTGGACTGACGCACCATTT

PI3K12

GGCGTAACATCCAGCTTTGC

GCTCCTGGAAGCTGGGTAAC

  1. Note: 1Akt: Protein kinase B; 2GS: Glycogensynthase; 3PEPCK: Phosphoenol pyruvate carboxykinase; 4IRS-1: Insulin receptor substrate 1; 5G6PDH: Glucose-6-phosphate dehydrogenase; 6GK: Glucokinase; 7PK: Pyruvate kinase; 8FAS: Fatty acid synthase; 9ACC: Acetyl CoA carboxylase; 10GLUT2: Glucose transporter 2; 11G6P: Glucose 6-phosphatase; 12PI3K: Phosphoenolpyruvate carboxykinase.
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