Figure 4

In vitro cytotoxicity experiments. (a) Relative viability of MCF-7 cells incubated with different concentrations of Fe3O4/ICG@PLGA/PFP NPs under laser irradiation (808 nm, 1 W/cm2, 5 min). (b) Fluorescence images of Hoechst 33342/PI co-stained MCF-7 cells incubated with Fe3O4/ICG@PLGA/PFP NPs (0.5 mg/mL) and exposed to the 808 nm laser irradiation (1 W/cm2, 5 min). Live and dead cells were stained with Hoechst 33342 (blue color) and PI (red color), respectively.