Figure 3
From: Generally Applicable Transformation Protocols for Fluorescent Nanodiamond Internalization into Cells
Survival of yeast cells after performing the interventions and different steps in the transformation protocols separately. Colony forming units (CFUs) are a measure for the survival of viable cells after either the chemical transformation or the electroporation protocol. (A) The greatest reduction of viability occurs after the addition of 0.01% Triton. The addition of FNDs to the treatment does not decrease the viability. Bars represent averages of triplicates out of two independent experiments. An area of +/−20% around 100% is deemed as ‘normal viability,’ (B). Electroporation reduces viability by a factor 102. In all samples FNDs were also added. In the case of 8 pulses, cell viability was completely reduced. For the electroporation protocol all decreases were significant (p < 0.0001). Bars represent averages of replicates out of two independent experiments, error bars show the Standard Error of the Mean. Significance is tested compared to the control situation. *p < 0.05, ***p < 0.001.
