Figure 1 | Scientific Reports

Figure 1

From: Sudan ebolavirus long recovered survivors produce GP-specific Abs that are of the IgG1 subclass and preferentially bind FcγRI

Figure 1

Production and activation of the BW-FcγR reporter cells by IgG. (a) Detection of stable transfected Fcγ Receptors was screened by flow cytometry using combined staining with anti-human CD-16/ 32/ 64 -black line, secondary APC- grey line. (b) Activation of BW reporter cells by commercial mAb. 96-well culture plates were pre-coated with Goat anti-mouse IgG (2 μg/ml), blocked, washed and coated with mAbs specific for each FcγR 250 ng/ml. Following additional washing, BW-FcγR cell lines were added, 50*103 cell/well. mIL-2 secretion was determined by ELISA kit after 16 h incubation. (c) IgG mediated activation process diagram of BW-FcγR reporter cells. Coating of plates with goat Fab2 portion anti human Fab2 of IgG, adding human sera containing IgG followed by seeding with reporter cells 50*103 cells/well. mIL-2 secretion of activated cells determined by ELISA after 16 h incubation. (d) Activation of BW reporter cells by control sera (1:5 K and 1:25 K dilution). mIL-2 secretion was determined by ELISA after 16 h incubation.

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