Figure 5
Nucleocytoplasmic translocation of HDAC9 is required for TC axon branching via MEF2 regulation. (a) Average number of branch points per axon. Thalamic cells were electroporated with only mCherry, HDAC9 co-electroporated with mCherry, nucHDAC9 co-electroporated with mCherry, and nucHDAC9ΔMEF2 co-electroporated with mCherry. N = 12, 17, 18 and 14 axons from 4–6 co-cultures for each condition, respectively. (b) Branch density per axon. (c) Total branch length per axon. (d) Branch tip length. Statistical analysis by Kruskal-Wallis test with Dunn correction comparing the 4 columns. *p < 0.05. ***p < 0.001. ****p < 0.0001. e. Laminar distribution of branching points according to their distance from the pial surface. Arrows indicate presumed layer 4. Statistical analysis by two-way ANOVA for whole column effects with Tukey’s multiple comparisons test confirmed a significant difference in laminar specificity between nucHDAC9 and control (p < 0.0001), while no significant difference was found between nucHDAC9ΔMEF2 and control or wild-type HDAC9.
