Figure 4

Percentage of bone formation within the implanted matrices over time of implantation. The quantification was performed from Trichrome Masson staining slides (n = 3) on the defects filled with the three groups of biomaterials over weeks 1, 3 and 5. Significant differences between biomaterials at each week are indicated with *p < 0.05 for week 3 and ^##p ≤ 0.01 for week 5. Of note, the percentage of bone formation obtained on the Matrix-HA-Fuco filled defects were significantly different to each other at each time point. The percentage obtained on the Matrix-HA filled defects at week 1 was significantly different to the ones obtained at week 3 and week 5. Similarly, the percentage obtained on the Matrix-Fuco filled defects at week 5 was significantly different to the ones obtained at week 1 and week 3.