Figure 3

Prestin molecular phylogenetic analysis by Maximum Likelihood method. Using the Maximum Likelihood method based on the best fitted models (LG + G), the tree with the highest log likelihood (−1660.211) is shown. A Gamma distribution (G) was used to model evolutionary rate differences among sites (5 categories ( + G, parameter = 0.79). The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 17 amino acid sequences with respective VectorBase accession number: A. albimanus (AALB009835); A. arabiensis (AARA008979); A. atroparvus (AATE014136); A. coluzzii (ACOM027477); A. culicifacies (ACUA008536); A. darlingi (ADAC002173); A. dirus (ADIR005276); A. epiroticus (AEPI009604); A. farauti (AFAF017372); A. funestus (AFUN006954); A. gambiae (AGAP010725); A. maculatus (AMAM007756); A. melas (AMEC018923); A. merus (AMEM004325); A. minimus (AMIN009235); A. stephensi (ASTE009391) and Ixodes scapularis (ISCW019766) (outgroup). All positions containing gaps and missing data were eliminated and bootstrap values for internal branches are shown.