Figure 4

NDAE1 molecular phylogenetic analysis by Maximum Likelihood method. Using the Maximum Likelihood method based on the best fitted models (LG + G), the tree with the highest log likelihood (−4016.814) is shown. A Gamma distribution (G) was used to model evolutionary rate differences among sites (5 categories ( + G, parameter = 0.27). The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 17 amino acid sequences with respective NCBI accession number: A. albimanus (AALB000104); A. arabiensis (AARA004033); A. atroparvus (AATE011952); A. coluzzii (ACOM026960); A. culicifacies (ACUA004881); A. darlingi (ADAC005721); A. dirus (ADIR005084); A. epiroticus (AEPI001894); A. farauti (AFAF017853); A. funestus (AFUN000817); A. gambiae (AGAP009736); A. maculatus (AMAM011081); A. melas (AMEC001882); A. merus (AMEM008317); A. minimus (AMIN009479); A. stephensi (ASTE003102) and Ixodes scapularis (ISCW000340) (outgroup). All positions containing gaps and missing data were eliminated and bootstrap values for internal branches are shown.