Figure 5

Results of prestinA and NDAE1 gene knockdown in Anopheles stephensi salivary glands. (A–C) The relative expressions of prestinA and NDAE1 were evaluated in Anopheles stephensi salivary glands with gene knockdown of prestinA, NDAE1, and in co-silenced groups, respectively. Normalized against RPS7, Cq values were compared between mosquitoes injected with dsRNA for the selected genes and with β2M control dsRNA. The expression of the genes in the prestinA, NDAE1 and both silenced groups is set to 1 for a better interpretation. Statistical analyses were conducted using the Pfaff method. *P < 0.05, **P < 0.01, ***P < 0.001. (D) Summary of silencing assay results. Gene expression levels and gene knockdown percentage of the treated groups.