Figure 4
From: Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization
GABARAP, GABARAPL1 and GABARAPL2 colocalize with Nef in vesicular structures. (A) Live-cell fluorescence microscopy of HEK293 cells stably expressing Nef-CFP. Here, Nef was observed at cell-cell contact sites, bleb-like structures or at the plasma membrane (PM). Scale bar 20 µm. (B) Live-cell fluorescence microscopy of HEK293 cells transfected with peFP-C1/GABARAP (top), peYFP-C1/GABARAPL1 (middle) and peYFP-C1/GABARAPL2 (bottom) revealed a diffuse distribution pattern for all GABARAPs. Scale bar 20 µm. (C) Live-cell fluorescence microscopy of HEK293 cells stably expressing Nef-CFP (red) and transfected with peFP-C1/GABARAP (top), peYFP-C1/GABARAPL1 (middle) and peYFP-C1/GABARAPL2 (bottom) (all green). In this case, Nef and GABARAPs showed high colocalization levels at vesicular structures and at the plasma membrane (see merge and zoom). Scale bar 20 µm.
