Figure 5
From: Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization
GABARAPs are required for plasma membrane localization of Nef. (A) Live-cell fluorescence microscopy of HEK293 cells stably expressing Nef-DsRed (red) and mock transfected as a control (top), transfected with scrambled siRNA (middle) or with a pool of GABARAP, -L1 and –L2 siRNAs (bottom). Hoechst 33442 was used to stain the nucleus (blue). Scale bar 20 µm. (B) Quantification of cells with Nef plasma membrane localization after treatment with the respective siRNAs from three independent experiments. **p < 0.05. The average value of three control experiments was set to 100%.
