Figure 5
Positional cloning of the ORB1 gene. (a) A mapping population of 684 F2 plants derived from an orb1-1 × Col-0 cross allowed us to define a candidate interval of 222 kb on chromosome 5. Names and physical map positions of the molecular markers used for linkage analysis are shown. The number of recombinant chromosomes found and the total of chromosomes analyzed are indicated in parentheses. (b) Electropherograms showing the point mutations in the orb1-1, orb1-2 and orb1-3 mutants. (c) Pileup of reads derived from the orb1-1 genome. The reference sequence is shown in green, and the orb1-1 mutation is highlighted in red. (d) Structure of the At5g04140.1 isoform of the ORB1 gene and alignment of RNA-seq read pairs to the At5g04140.1 isoform, with indication of the nature and position of the orb1 mutations. Boxes and lines between boxes indicate exons and introns, respectively. White boxes represent the 5′- and 3′-UTRs. A triangle indicates the T-DNA insertion in orb1-4.
