Figure 8

Differences in the expression of genes encoding glutamate-related enzymes and components of the cytosolic ribosome between Ler and orb1-3 plants. Expression levels of genes encoding (a) glutamate synthases (Fd-GOGAT1, Fd-GOGAT2 and NADH-GOGAT), (b) glutamine synthetases (GLN1.1, GLN1.2, GLN1.3, GLN1.4, GLN1.5 and GLN2), (c) asparagine synthetases (ASN1, ASN2 and ASN3), (d) glutamate dehydrogenases (GDH1, GDH2 and GDH3), and (e) several components of the cytosolic ribosome in Ler and orb1-3 plants, expressed as FPKM (fragments per kilobase of transcript per million fragments mapped). Error bars indicate standard deviations. Asterisks indicate values significantly different from Ler as determined by Cuffdiff (*q < 0.05, ***q < 0.001, n = 3). The expression levels of the genes encoding components of the cytosolic ribosome were normalized based on the mean and standard deviation of the FPKM values obtained for each gene in all samples analyzed.