Figure 3

In situ hybridization analysis of miR-15b expression using a digoxigen-labeled locked nucleic acid antisense miR-15b probe in frozen sections of the injured rat brain. miR-15b is highly expressed in many but not all dying, Fluoro-Jade-positive (FJ+) neurons in the hippocampal CA1-3 subfields, in all dying (FJ+) neurons of the dentate gyrus (DG) (A), and in all FJ+ neurons of the cortex (B).