Table 1 List of plasmids used in this work.
Plasmid | Description | Selection Marker | Source of reference |
|---|---|---|---|
pGEX-5X-GALNSopt | pGEX-5X-3 plasmid encoding the enzyme N-acetylgalactosamine-6-sulfatase codon-optimized for expression in E. coli | AmpR | This study |
pGEXosmY | Modification of the pGEX-5X-GALNSopt plasmid where the promoter tac was replaced by the osmY promoter with its corresponding RBS | AmpR | This study |
pGEXproUmod | Modification of the pGEX-5X-GALNSopt plasmid where the promoter tac was replaced by the proU mod promoter with its corresponding RBS | AmpR | This study |
pACYCDuet™-1 | Bicistronic plasmid used as a control and to clone all the chaperones used in this study | CmR | Novagen, Merck Milipore |
pDuet::GroS | Plasmids for the overexpression of the genes groS, groL, groS and groL, dnaK, dnaJ, dnaK and dnaJ, ibpA, ibpB, ibpA and ibpB, dsbA, dsbB, dsbA and dsbB, grpE, and clpB (respectively) driven by the lac promoter. Backbone pACYCDuet™-1 | CmR | This study |
pDuet::GroL | |||
pDuet::GroSL | |||
pDuet::DnaK | |||
pDuet::DnaJ | |||
pDuet::DnaKJ | |||
pDuet::IbpA | |||
pDuet::IbpB | |||
pDuet::IbpAB | |||
pDuet::DsbA | |||
pDuet::DsbB | |||
pDuet::DsbAB | |||
pDuet::GrpE | |||
pDuet::ClpB |
