Figure 1 | Scientific Reports

Figure 1

From: Fasciclin-like arabinogalactan proteins, PtFLAs, play important roles in GA-mediated tension wood formation in Populus

Figure 1

PtFLA6 expression analyses during TW formation. (a) TW induction by mechanical stress. Two-month-old wild type poplar plants (Shanxin yang) grown in greenhouse were bended for two weeks. The black vertical bar indicates the part to be taken for analyses. Scale bar = 1 cm. (b) Sections of bended stems were stained with safranin-O and astra-blue. TW was stained into blue. The horizontal line indicates the division between TW side and OW side. TW, tension wood; OW, opposite wood. Scale bar = 1 mm. (c) Western blotting analysis of PtFLA6 protein during TW formation. A total amount of 15 µg proteins was separated by SDS-PAGE and hybridized with anti-PtFLA6 antibodies (Anti-FLA6) or plant actin antibodies (Anti-Actin). FLA6 + GST, fusion protein for antibody production; OW-X, xylem tissues in the OW side; OW-B, the bark tissues of OW side; TW-X, xylem tissues in the TW side; TW-B, the bark tissues of TW side. (d) PtFLA6 promoter activity analysis during TW formation. Sections from the bended site of PtFLA6 promoter-GUS transgenic plants were used for GUS staining. Scale bar = 1 mm.

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