Figure 3

Immunocytolocalization of PtFLA6 in the G-fiber cells of TW xylem tissues. Transverse ultra thin sections of the xylem in the TW side were used for immunogold labeling TEM analysis. (a,d,g) Accumulation of PtFLA6 protein was examined in the differentiating G-fibers (a), the mature G-fibers (d) and the negative control (g), respectively. (b,e,h) Higher magnification images of the framed areas in (a,d,g), respectively. PtFLA6 labeling (black dots) was mainly detected in the differentiating G-fibers compared to that in the mature G-fibers. No labeling was seen in the control. (c,f,i) Higher magnification images of the framed areas in (b,e,h). PW, primary wall; SW, secondary wall; G, G-layer. Scale bars = 250 nm.