Figure 6
TW formation was inhibited by daminozide treatment. (a) Cross-sections of bended stems treated with daminozide or lanolin alone. The horizontal line indicates the division between TW side and OW side. TW, tension wood; OW, opposite wood. Scale bars = 1 mm. (b) Radio of TW area in the xylem of bended stems treated with daminozide. Error bars represent the SDs from five plants. (c) Expression analysis of PttGA20ox1 in the TW side of bended stems treated with daminozide. (d) GA3 content in the TW side of daminozide treated stems. (e) Western blotting analysis of the poplar DELLA protein and PtFLA6 protein in the xylem of the TW side. An amount of 30 μg plant proteins extracted from the TW xylem tissues of bended stems treated with daminozide or lanolin were separated by 10% SDS-PAGE and hybridized with AtRGA1 antibodies (Anti-RGA1), PtFLA6 antibodies (Anti-FLA6) or plant actin antibodies (Anti-Actin). (f) qRT-PCR analysis of PtFLAs in the TW xylem tissues of bended stems treated with daminozide. Gene expression level in control was set to 1. Error bars represent the SDs from three biological replicates. Control, bended stems painted with lanolin alone; D, bended stems painted with daminozide. **Indicates a significant difference in comparison to control at P < 0.01 (Student’s t-test).
