Figure 7
PtRGA1 negatively regulates the expression of PtFLAs. (a) Diagrams of effector and reporter constructs for transactivation studies. (b) Transactivation assays. Promoter activities of PtFLA1, PtFLA6 and PtFLA9 were obviously repressed in N. benthamiana leaves infiltrated with the construct combinations of Pro35S::PtRGA1 and each ProPtFLA::LUC. Bars = 1 cm. (c,d) Expression analysis of PtFLAs in the poplar xylem protoplasts overexpressing PtRGA1. RNA was extracted from the poplar xylem protoplasts transfected with pGreenII62-SK-PtRGA1. Then qRT-PCR was performed to analyze the transcriptional levels of PtRGA1 (c) and PtFLAs (d). Gene expression level in control was set to 1. Error bars represent the SDs from three biological replicates. Control, protoplasts transfected with pGreenII62-SK; PtRGA1, protoplasts transfacted with pGreenII62-SK-PtRGA1.
