Figure 2 | Scientific Reports

Figure 2

From: Effects of 4(1H)-quinolinone derivative, a novel non-nucleotide allosteric purinergic P2Y 2 agonist, on cardiomyocytes in neonatal rats

Figure 2

Compound 89 exhibits allosteric modulatory effects on ATP-induced Ca2+ mobilization in P2Y2-overexpressing 1321N1 cells and in cardiomyocytes. (a) Concentration-response curves of physiological agonists in a FLIPR Ca2+ mobilization assay using hP2Y2 stably-expressing 1321N1 cells and NRCMs. (b) Allosteric modulatory effect of Compound 89 on the ATP concentration-response curve (left), and allosteric modulatory effect of ATP on the Compound 89 concentration-response curve (right) in hP2Y2 stably-expressing 1321N1 cells. (c) Allosteric modulatory effect of Compound 89 on the ATP concentration-response curve (left), and the allosteric modulatory effect of ATP on the Compound 89 concentration-response curve (right) in NRCMs. The activity of 10 μM ATP was normalized to 100%. All data points represent the mean of experiments performed in duplicate or triplicate, and comparable results were obtained by another independent experiment.

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