Figure 2
BMP9-induced osteogenic differentiation of rDFCs. (A) ALP staining intensity was enhanced in BMP9 group when compared with the blank group and GFP group. Bars: 50 μm. (B) ALP activity was also significantly increased on days 3, 5, 7, 9 in BMP9 group when compared with the blank group and GFP group. (C) Calcium deposition was stained by Alizarin red staining. More calcified nodules were found in BMP9 group when compared with the blank group and GFP group. Bars: 50 μm. (D,E and F) the relative expression of ALP, OPN, and Osterix were detected by Real Time-qPCR. *P < 0.05, **P < 0.01, ***P < 0.001, compared with blank group, #P < 0.05, ##P < 0.01, ###P < 0.001, compared with GFP group.
