Figure 5 | Scientific Reports

Figure 5

From: In vivo imaging to monitor differentiation and therapeutic effects of transplanted mesenchymal stem cells in myocardial infarction

Figure 5

Characterisation of the MI model and ex vivo analysis of rat myocardial tissue 45 days after MI. (A) Haematoxylin and eosin staining of myocardium from the sham-operated (G1), MI model (G2), and transfected-BMSC-treated MI model (G3). Scale bar, 50 μm. (B) Haematoxylin and eosin staining of heart tissue sections from the G3 group. Arrows indicate BMSCs. (C) HSV1-tk (red) and DAPI (blue) immunohistochemical staining of rat heart tissue from the G3 4 days after treatment. Arrows indicate overlapping (i.e. positive) staining of transplanted labelled BMSCs. Scale bar, 30 μm. (D) Immunostaining for GATA-4 and cardiac troponin I (cTnI) (E) in myocardium derived from α-MHC-induced differentiation of BMSCs. Scale bar, 50 μm. (F) Immunocytochemical analysis of HSV1-tk (red) and the cardiomyocyte-specific marker cTnI (green), and DAPI staining (blue), and their colocalisation in heart tissue from the G3 45 days after transplantation. Scale bar, 50 μm.

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