Figure 3
From: Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis
Histological alterations in skeletal muscle. (A) Representative sections of tibialis anterior (TA) of control (left) and AiA (right) rabbits. Carazzi haematoxylin and eosin staining. Scale bar = 100 μm. (B) Representative sections of type I (white) and II (black) fiber distribution and size in TA of control (left) and AiA (right) animals stained with ATPase pH 9.4. Scale bar = 100 μm. (C) Representative TA cross-section with RAM11 immunostaining of control (left) and AiA (right) groups. Scale bar = 100 μm. Inset showed RAM11 immunoreactive cell (black arrow) beneath the epimysium in AiA. Scale bar = 25 μm. (D–F) Representative focal microscopy images from segments of extensor digitorium longus (EDL) fibers in control (D) and AiA rabbits (E). Rhodamine phalloidin (red) was used to stain actin and DAPI (blue) to stain nuclei. Scale bar = 30 µm. (F) Myonuclei number in control in comparison to AiA group expressed as number of nuclei per 100 mm of fiber segment.
