Figure 2 | Scientific Reports

Figure 2

From: Mass Spectrometry Imaging proves differential absorption profiles of well-characterised permeability markers along the crypt-villus axis

Figure 2

Longitudinal (proximal to distal) and crypt-villus distribution of test compounds in rat small intestine. Images and concentration determinations from samples collected 20 min post oral administration (1 µmol/kg) at different distances distal from the pyloro-duodenal transition. (a) MALDI MSI ion distribution images of propranolol, metoprolol, and atenolol in intestine, scale bar is 1 mm. All tissue sections were analysed at the same occasion and each compound is displayed with its own relative intensity scale (rainbow). Propranolol intensity is scaled to 10% of max intensity, metoprolol intensity is scaled to 10% of max intensity, and atenolol intensity is scaled to 7% of max intensity. (b) Homogenate concentrations measured by LC-MS/MS for the three investigated compounds; propranolol, metoprolol, and atenolol. The graphs present mean concentrations and range of duplicate determinations, in some instances error bars are shorter than height of symbols. X-error bars indicate position of the sampling +/− 1 cm distal from the pyloro-duodenal transition.

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