Figure 3
PCA analyses of T7RNAP at three different pH values depicting the motions along the first five, the tenth, and the twentieth eigenvectors during the 100 ns simulation run and FEL analyses of T7RNAP depicting low energy basin along with the representative structure retrieved at each pH. (a) pH 7.9, (b) pH 5 and (c) neutral pH. SPA analysis on T7RNAP at pH 7.9 and 5 showing sections of TEM micrographs of negatively stained T7RNA (d) at pH 7.9 and at (f) pH 5 (inset shows the representative views of the average of several particles – not to scale) with their respective 3D reconstruction views (e) and (g) respectively.
