Figure 4

Domain motion analysis showing thumb sub-domain movement of T7RNAP as it transitions from pH 5Â (a) to pH 7.9Â (b) as identified by Dyndom. The hinge regions are colored red and the moving domains are colored green. The direction of movement along the hinge axis is depicted by the pink arrow. Porcupine plots showing prominent motions with directions at (c) pH 5 and (d) pH 7.9.