Table 1 In vivo estimate of heme crystallization rate.

From: Unraveling heme detoxification in the malaria parasite by in situ correlative X-ray fluorescence microscopy and soft X-ray tomography

Parasite

#Fe (Hz) (×108)

# nuclei

Stage # (# nuclei)

Age range [h]

Age ± esd [h]

Rate of Hz crystallization [heme/s]

Onset of Hz crystallization [h]

1

1.84

1 or 2

6 (1–2)

34–38

36 ± 1.6

6900 ± 2300

23.7 ± 0.7

2

2.92

1 or 2

6 (1–2)

34–38

36 ± 1.6

3

3.02

1(2)

6 (1–2)

34–38

34 ± 1.9

4

3.78

4

7 (3–5)

38–44

41 ± 2.4

5

4.03

7

8 (6–~16)

44–48

44.4 ± 1.8

6

4.37

5

7 (3–5)

38–44

44 ± 2.8

7

7.67

≥12

8 (6–~16)

44–48

46.4 ± 1.6

8

2.82

1

5 (1)

30–34

32 ± 1.6

3.02

3.03

  1. Shown in the 2nd column are the total amounts of Fe atoms measured in the hemozoin crystals for the eight different parasites. The axial and in-plane alignment procedure was used to obtain the amounts of Fe for parasites 1–7, and for parasite 8 in its top row. The second Fe estimate for parasite 8 (middle row) was obtained from the 3D segmentation and fluorescent modeling alignment, and the third estimate (bottom row) from the volume of the crystals was obtained directly from the soft X-ray tomography (SXT) image. Note that the three different estimates for parasite 8 are close. The number of nuclei per parasite was obtained directly from the SXT image, and then converted to a stage based on published criteria26. Age was then estimated from this stage as described in Methods. Iron content vs. age were then plotted (Fig. 5), and the slope and y intercept of the best fitting line yielded the estimates for the heme crystallization rate and the onset of hemozoin (Hz) crystallization.
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