Figure 5
From: Skin renewal activity of non-thermal plasma through the activation of β-catenin in keratinocytes
The effect of NTP on β-catenin activity in skin tissue. (a) Marked regions on the dorsal skin of four HRM2 mice were subjected to NTP treatment for 5 minutes at the indicated time point (0, 3, 6, and 24 hours) before sacrificing the mice. (Left panel) The skin tissue was isolated and subjected to immunofluorescence staining of β-catenin (red), DAPI counterstaining of the nuclei (blue), and confocal imaging. Scale bar: 20 μm. (Right panel) The graph showing the percent of nuclear β-catenin positive cells in epidermal tissues, *p < 0.001. (b) Non-treated and NTP-treated skin tissue was isolated 24 hours after treatment and subjected to immunohistochemistry against cyclin A and E. The nuclei were counterstained using hematoxylin. Scale bar: 100 μm. The number of Cyclin A and Cyclin E-positive cells were counted and represented as graphs (Right panels). Data shown are representative of three independent experiments, *p < 0.05.
