Table 2 Clinical isolates analyzed in the study.

From: Discovery of monoclonal antibodies cross-reactive to novel subserotypes of K. pneumoniae O3

Strain

Country of origin

Resistance profilea

MLST

Beta-lactamase

Specimen

PCR

Immunoblot

wbdA sequencing

Serotype

Accession number

K-typec

Pan-O3

O3/O3a

O3b

2F8

1G6

Kp2

Hungary

ESBL

ST1915

nd

urine

+

+

+

+

C at position 80

O3

SRR5270320

K60

Kp14

Hungary

ESBL

ST113

SHV

central venous route

+

+

+

+

C at position 80

O3

SRR5270322

K35

Kp18

Hungary

ESBL

ST834

CTX-M

blood culture

+

+

+

 

O3b

SRR5270321

Non typeable

Kp28

Hungary

ESBL

ST323

SHV

central venous route

+

+

+

 

O3b

SRR5270319

K51

Kp35

Poland

ESBL, CR

ST11

CTX-M-3, SHV-12, KPC-2

na

+

+

+

 

O3b

SRR5270318

Non typeable

Kp49

Spain

CR

ST54

VIM-1

urine

+

+

+

 

O3b

 

nd

Kp62

Spain

CR

ST384

KPC-3

urine

+

+

+

+

C at position 80

O3

SRR5270317

K35

Kp72

Spain

ESBL

ST14

TEM-4

na

+

+

+

 

O3b

 

nd

Kp77

Spain

ESBL

ST385

SHV-12

urine

+

+

+

 

O3b

SRR5270316

Non typeable

Kp81

Spain

ESBL

ST346

SHV-12

wound

+

+

+

 

O3b

SRR5270315

Non typeable

Kp82

Spain

ESBL

ST370

SHV-12

wound

+

+

+

 

O3b

SRR5270314

K28

Kp83

Spain

ESBL

ST346

SHV-12

rectal swab

+

+

+

 

O3b

 

nd

Kp100

Spain

ESBL

ST389

CTX-M-10

urine

+

+

-

+

+

C at position 80

O3

SRR5270326

Non typeable

Kp102

Spain

ESBL

ST34

CTX-M-10

respiratory origin

+

+

+

 

O3b

SRR5270325

K38

Kp103

Spain

ESBL

ST389

CTX-M-10

urine

+

+

+

+

C at position 80

O3

 

nd

Kp105

Spain

ESBL

ST16

CTX-M-15

urine

+

+

+

 

O3b

 

nd

Kp107

Spain

ESBL

ST16

CTX-M-15

urine

+

+

+

 

O3b

SRR5270324

K51

Kp116

UAE

CR

ST1798

OXA-48-like

sputum

+

+

 

non reactive b

SRR5270323

K58

Kp184

UAE

CR

nd

NDM

blood culture

+

+

+

+

C at position 80

O3

 

nd

Kp236

UAE

 

nd

blood culture

+

+

+

 

O3b

 

nd

Kp237

UAE

 

nd

blood culture

+

+

+

 

O3b

 

nd

Kp245

UAE

 

nd

blood culture

+

+

+

 

O3b

 

nd

Kp257

UAE

 

nd

blood culture

+

+

+

+

C at position 80

O3

 

nd

Kp269

Israel

CR, ESBL

nd

nd

blood culture

+

+

+

 

O3b

 

nd

Kp292

Italy

CR, ESBL, colR

ST273

KPC-3

na

+

+

+

 

O3b

 

nd

Kp345

Italy

CR

ST273

KPC-3

na

+

+

+

 

O3b

 

nd

LA-KP28

USA

 

nd

nd

endotracheal aspirate

+

+

+

+

C at position 80

O3

 

nd

LA-KP38

USA

ESBL

nd

nd

endotracheal aspirate

+

+

+

+

C at position 80

O3

 

nd

FSJ28

Germany

ESBL

nd

nd

urine

+

+

+

 

O3b

 

nd

FSJ29

Germany

ESBL

nd

nd

urine

+

+

+

 

O3b

 

nd

FSJ48

Germany

ESBL

nd

nd

urine

+

+

+

 

O3b

 

nd

  1. aOnly ESBL production (ESBL), carbapenem resistance (CR) and colistin resistance (colR) indicated.
  2. bDespite a low amount of high-molecular weight polysaccharide detected by silver staining, no reactivity with any of the mAbs tested.
  3. cCapsule type (K-type) of whole-genome sequenced isolates based on in silico prediction. nd - not determined. na - not available.