Figure 2
Optical coherence tomography enables circular scans of the retina to detect changes of the retinal nerve fibre thickness. The optical coherence tomography (OCT) enables cross-sectional images of the retina in vivo to gather longitudinal information about changes of the retinal nerve fiber layer thickness (RNFLT). RNFLT was defined as the distance between the inner limiting membrane (ILM, red line) and the retinal nerve fiber layer (RNFL, green line). For orientation, the basal membrane (BM, lower red line), indicating the border to the retinal pigment epithelium, is shown. (a) Using the Heidelberg Eye Explorer software, the native segmentation algorithm designed to identify specific layers of the human retina, was not always suitable for rodent retina. The RNFL needed manual correction with the circle device. (b) The baseline measurement of a CTRL animal. (c) The follow-up of the same eye at the end of the study shows distinct changes of the RNFLT as indicated by arrows. (d) The loss of RNFLT at the end of the study was compared with after IOP elevation. Fellow eyes were not affected. IOP elevated eyes of CTRL, CTRL IgG and Buffer group showed a significant loss, while damage was less severe in α-synuclein Ab group. With respect to α-synuclein Ab group, RNFLT was significantly higher than in CTRL, CTRL IgG and Buffer groups. **Represents p < 0.01 compared with fellow eyes. ##Represents p < 0.01 compared with IOP elevated eyes of α-synuclein Ab group. ns = not significant.
