Figure 5

Vascular ER stress and endothelial dysfunction in mice due to conditional deletion of endothelial Sirt1 is mediated by miR-204. In situ hybridization with quantification for miR-204 (A,D), immunofluorescence with quantification for BIP (B,E), and endothelium-dependent relaxation in thoracic aortas (A–C) and MRAs (D–F) of EC Sirt1^−/− and Sirt1^flx/flx mice transfected ex vivo with miR-204 I or miR SC. n = 3. Values expressed relative to Sirt1^flx/flx + miR SC. *p < 0.05 vs Sirt1^flx/flx + miR SC, Sirt1^flx/flx + miR-204 I, and EC Sirt1^−/− + miR-204 I.