Figure 6

MiR-204 promotes and Sirt1 suppresses ER stress-induced vascular reactive oxygen species. Immunofluorescence and quantification for endothelial 8-hydroxydeoxyguanosine (8-OHdG) in MRAs of C57Bl/6 mice systemically infused with miR-204 I and treated with Tunica or Veh (A), and EC Sirt1^−/− or Sirt1^flx/flx mice treated with Tunica or Veh (B). Values expressed relative to Sirt1^flx/flx + Veh. miR SC + Veh: white bar, miR SC + miR-204 I: black bar, miR SC + Tunica: grey bar, miR-204 I + Tunica: checkered bar. *p < 0.05 vs miR SC + Veh, and miR-204 I + Veh (in A), and Sirt1^flx/flx + Veh (in B). ^p<0.05 vs miR SC + Tunica. #p<0.05 vs EC Sirt1^−/− + Veh. n = 3.