Figure 5 | Scientific Reports

Figure 5

From: JMJD3 and NF-κB-dependent activation of Notch1 gene is required for keratinocyte migration during skin wound healing

Figure 5

Notch1 activates RhoU and PLAU gene expression in scratch-wounded keratinocytes. (a) ChIP assay was performed in scratch-wounded HaCaT keratinocytes using the anti-NICD and anti-acetylated H3 antibodies, respectively. The occupancy of each protein was determined with quantitative PCR at the RhoU and PLAU gene promoter region encompassing the RBP-J binding site. ChIP using normal IgG was performed as a negative control. (b~d) Inactivation of Notch1 (DAPT) or JMJD3 (GSK-J4) or NF-κB (BAY 11–7082) results in decreased recruitment of NICD to the RhoU and PLAU gene promoters. Following DAPT, GSK-J4, or BAY11–7085 treatment, HaCaT keratinocytes were scratched and ChIP assay was performed.

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