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Figure 1

From: In vivo genetic manipulation of inner ear connexin expression by bovine adeno-associated viral vectors

Figure 1

In vivo fluid delivery via canalostomy to mouse cochlea. (a,b) Midmodiolar cochlear sections from P25 (a) and P4 (b) mice injected with fluorescent wheat germ agglutinin (which labels cell membranes) examined by confocal microscopy; scale bars: 50 μm. Insets: representative light microscopy images of cochleae from P25 (a) or P4 (b) mice injected with bromophenol blue, dissolved in PBS; images were acquired 5 min after canalostomy; scale bars: 500 μm; arrowheads point to the base (black) and apex (white) of the cochlea. BM: basilar membrane; GER, greater epithelial ridge; IHC: inner hair cell; IS: inner sulcus; LER, lesser epithelial ridge; LM: spiral limbus; OHC: outer hair cells; OS: outer sulcus; OSL, osseous spiral lamina; RM: Reissner’s membrane; SL: spiral ligament; SV: stria vascularis; TM: tectorial membrane. (c) Hearing thresholds determined by analysis of auditory brainstem responses (ABR) in wild type mice injected at P25 (red), P4 (cyan) and non-injected controls (black); ordinates are sound pressure levels (SPL) relative to 20 µPa: Error bars represent standard error of the mean (s.e.m.).

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