Figure 3
From: Glucocorticoid therapy regulates podocyte motility by inhibition of Rac1
The GR cistrome. GR-binding sites (GBS) in human wild type podocytes were identified using ChIP-Seq and were analysed using the cis-regulatory element annotation system (CEAS). (a) Illustrates the percentage of GBS located in different functional genomic units. (b) The relation between GBS and the transcriptional output elicited by Gc-exposure identified by microarray was compared. The large pie chart shows division of GBS according to distance from Gc-regulated genes. Linked diagrams shows the percentage of GBS sites found in areas surrounding Gc –responsive and –unresponsive genes. Bar chart shows GBS enrichment in 2.5 kb promoter region over general genome background for Gc –responsive and –unresponsive genes. (c) Results of de novo motif enrichment on GR ChIP-Seq peaks using HOMER. The size of the letter for each nucleotide position relates to how dominant the base is for that position.
