Figure 5
From: Glucocorticoid therapy regulates podocyte motility by inhibition of Rac1
Early effects of Gc on podocyte motility. To analyse the length of time taken for Gc to affect podocyte speed, live cell imaging of wild type podocytes was performed in the 24 hour period following treatment. Each experiment consisted of manually tracking 120 cells per condition using ImageJ software. The experiment was performed twice. (a) Shows the mean cell speed during the whole period of imaging. (b) Shows the instantaneous speed for the podocytes during the 24 hour period. Coloured arrows on the X-axis refer to the level of significance of the difference between the means of cell speed between vehicle- and Gc-treated cells. The first time of significant difference is shown by the arrow at 120 minutes. Results were analysed by two-way repeated measures ANOVA followed by Šídák’s multiple comparisons test. * = adjusted p value 0.0134; *** = adjusted p value 0.0004; **** = adjusted p value < 0.0001. Error bars represent standard error of the mean.
